Considering that SSRs have been traditionally isolated from genomic libraries enriched in repetitive sequences, they are usually treated as neutral markers (Goldstein and Schlötterer 1999 Ellegren 2004). Microsatellites are frequently used for orphan plants (e.g., those without a publicly available reference genome sequence) and for polyploid and highly heterozygous species because of their codominant nature. SSRs are DNA sequences with a simple core motif of one to six nucleotides that is tandemly repeated. The diffusion of the SSRs in genetics is also due to their good transferability across similar species (Mnejja et al. 2019), being multiallelic and highly polymorphic. Microsatellites, also known in plant genetics as simple sequence repeats (SSRs), are one of the most suitable markers for Prunus diversity (Aranzana et al. 2015), and discrimination of clones (Gharbi et al. 2010), landraces examination (Manco et al. 2011), phylogenetic relationships (Liu et al. 2004), population structure analysis (Horvath et al. Moreover, DNA markers have been used in plum for different purposes, such as map-based cloning (Claverie et al. domestica has a high level of diversity (Zhebentyayeva et al. These analyses also confirmed that the European plum clade of P. 2019).Ĭonsiderable progress in describing and classifying the ample diversity of the European plum has been achieved with the introduction of DNA molecular markers (Decroocq et al. Cultivated varieties are typically classified in different pomological groups by breeders and retailers, although the degree of overlap of morphological traits and the complex interspecific origin of the species do not always allow a clear distinction among all the different groups (Zhebentyayeva et al. Genodive for polyploids skin#Commercially available European plum varieties present a range of phenotypic traits (e.g., fruits shape and size skin and flesh colour firmness taste) that originated several classifications. Further uses include the production of juices, fruit brandy, and flavours for jams, candies, sweets, and other baked foods. These are mainly marketed fresh, canned or dried (Neumüller 2011). The differences between SSR classes are discussed considering the neutral and non-neutral evolution, and the polyploidy and asexual propagation of the cultivated tree varieties.Įuropean plum ( Prunus domestica L.) is an economically important stone fruit crop, globally cultivated in temperate areas for its fleshy fruits. The two SSR classes provide complementary information in European plum, making the contribution of EST-SSRs useful not only as non-neutral markers. Moreover, the two classes of SSRs gave different, weakly correlated, estimations of distance-based parameters with gSSRs being more powerful for discriminating purposes. The analysis of an ample set of morphologically diverse varieties indicated that gSSRs and EST-SSRs provide different estimates of some of the locus-based indicators of diversity. The aim of this study was to compare the features and usefulness of genomic SSR (gSSR) and EST-SSRs in European plum ( Prunus domestica L.), an economically important, hexaploid stone fruit crop globally cultivated to produce fleshy fruits and derived foodstuff. Simple sequence repeats (SSRs) are among the most useful DNA markers in plant science.
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